Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?
Identifieur interne : 001420 ( Main/Exploration ); précédent : 001419; suivant : 001421Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?
Auteurs : Roslyn M. Bill [Royaume-Uni]Source :
- Journal of Pharmacy and Pharmacology [ 0022-3573 ] ; 2015-03.
Abstract
Objectives: Recombinant protein subunit vaccines are formulated using protein antigens that have been synthesized in heterologous host cells. Several host cells are available for this purpose, ranging from Escherichia coli to mammalian cell lines. This article highlights the benefits of using yeast as the recombinant host. Key findings: The yeast species, Saccharomyces cerevisiae and Pichia pastoris, have been used to optimize the functional yields of potential antigens for the development of subunit vaccines against a wide range of diseases caused by bacteria and viruses. Saccharomyces cerevisiae has also been used in the manufacture of 11 approved vaccines against hepatitis B virus and one against human papillomavirus; in both cases, the recombinant protein forms highly immunogenic virus‐like particles. Summary: Advances in our understanding of how a yeast cell responds to the metabolic load of producing recombinant proteins will allow us to identify host strains that have improved yield properties and enable the synthesis of more challenging antigens that cannot be produced in other systems. Yeasts therefore have the potential to become important host organisms for the production of recombinant antigens that can be used in the manufacture of subunit vaccines or in new vaccine development.
Url:
DOI: 10.1111/jphp.12353
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001880
- to stream Istex, to step Curation: 001880
- to stream Istex, to step Checkpoint: 000131
- to stream Main, to step Merge: 001422
- to stream Main, to step Curation: 001420
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?</title><author><name sortKey="Bill, Roslyn M" sort="Bill, Roslyn M" uniqKey="Bill R" first="Roslyn M." last="Bill">Roslyn M. Bill</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F6E11952D083724F50B9D5C02407D6ED37EEEB39</idno><date when="2015" year="2015">2015</date><idno type="doi">10.1111/jphp.12353</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-KM1XJXHK-3/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001880</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001880</idno><idno type="wicri:Area/Istex/Curation">001880</idno><idno type="wicri:Area/Istex/Checkpoint">000131</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000131</idno><idno type="wicri:doubleKey">0022-3573:2015:Bill R:recombinant:protein:subunit</idno><idno type="wicri:Area/Main/Merge">001422</idno><idno type="wicri:Area/Main/Curation">001420</idno><idno type="wicri:Area/Main/Exploration">001420</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?</title><author><name sortKey="Bill, Roslyn M" sort="Bill, Roslyn M" uniqKey="Bill R" first="Roslyn M." last="Bill">Roslyn M. Bill</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Life and Health Sciences, Aston University, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region></placeName></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Royaume-Uni</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Pharmacy and Pharmacology</title><title level="j" type="sub">Enhancing Vaccine Design Strategies. Guest Editors: Yvonne Perrie, Helen R. Griffiths and David Jones</title><title level="j" type="alt">JOURNAL OF PHARMACY AND PHARMACOLOGY</title><idno type="ISSN">0022-3573</idno><idno type="eISSN">2042-7158</idno><imprint><biblScope unit="vol">67</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="319">319</biblScope><biblScope unit="page" to="328">328</biblScope><biblScope unit="page-count">10</biblScope><date type="published" when="2015-03">2015-03</date></imprint><idno type="ISSN">0022-3573</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3573</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Objectives: Recombinant protein subunit vaccines are formulated using protein antigens that have been synthesized in heterologous host cells. Several host cells are available for this purpose, ranging from Escherichia coli to mammalian cell lines. This article highlights the benefits of using yeast as the recombinant host. Key findings: The yeast species, Saccharomyces cerevisiae and Pichia pastoris, have been used to optimize the functional yields of potential antigens for the development of subunit vaccines against a wide range of diseases caused by bacteria and viruses. Saccharomyces cerevisiae has also been used in the manufacture of 11 approved vaccines against hepatitis B virus and one against human papillomavirus; in both cases, the recombinant protein forms highly immunogenic virus‐like particles. Summary: Advances in our understanding of how a yeast cell responds to the metabolic load of producing recombinant proteins will allow us to identify host strains that have improved yield properties and enable the synthesis of more challenging antigens that cannot be produced in other systems. Yeasts therefore have the potential to become important host organisms for the production of recombinant antigens that can be used in the manufacture of subunit vaccines or in new vaccine development.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Midlands de l'Ouest</li></region><settlement><li>Birmingham</li></settlement></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Bill, Roslyn M" sort="Bill, Roslyn M" uniqKey="Bill R" first="Roslyn M." last="Bill">Roslyn M. Bill</name></region><name sortKey="Bill, Roslyn M" sort="Bill, Roslyn M" uniqKey="Bill R" first="Roslyn M." last="Bill">Roslyn M. Bill</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001420 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001420 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:F6E11952D083724F50B9D5C02407D6ED37EEEB39
|texte= Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?
}}
| This area was generated with Dilib version V0.6.33. |  |